The in-situ stress distribution of Manan glutenite reservoir is highly heterogeneous,and it is urgent to establish an in-situ stress interpretation model suitable for the heterogeneous glutenite reservoir to guide the design of fracturing stages and clusters. In this paper,by conducting rock mechanics and in-situ stress experiments using downhole cores,we obtained the conversion relationship between dynamic and static mechanical parameters of the glutenite reservoir,established the combined spring in-situ stress model for Manan glutenite reservoir and determined the optimal values of key parameters. Based on the in-situ stress calculation results,an unsupervised clustering model of the horizontal principal stress difference was built for the horizontal wellbore,and the decimeter-scale horizontal principal stress difference distribution and clustering categories were obtained. With comprehensive consideration of the inter-fracture stress interference,casing collar position,bridge plug position and in-situ stress clustering results,perforation clusters in a stage were arranged where the horizontal principal stress difference is within the stage average±10%. An optimization method for horizontal well fracture placement in glutenite reservoirs was developed and a computer algorithm was coded. The results show that the in-situ stress calculation error of the combined spring model is 0.7%-1.5%;the standard deviation of the vertical stress of the reservoir is 0.45,with significant heterogeneous distribution characteristics. In-situ stress interpretation and unsupervised clustering fracturing stage/cluster optimization were carried out for a typical glutenite reservoir horizontal well in Manan block. There are 12 categories identified by in-situ stress clustering,and the optimal number of fracturing stages is 17. The perforation clusters within a stage are placed in positions with similar horizontal principal stress differences,which is expected to improve the conformity and effectiveness of fracturing stimulation.